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SV40轉(zhuǎn)染人成骨細(xì)胞;hFOB 1.19
貼壁生長
在0.6mg/ml新霉素G418存在下,用溫度敏感的表達(dá)載體pUCSVisA58轉(zhuǎn)染自然流產(chǎn)的胎兒四肢組織,建立了此細(xì)胞系。 在許可溫度33.5oC下細(xì)胞分裂很快,SV40轉(zhuǎn)染人成骨細(xì)胞;hFOB 1.19而在限制溫度39.5oC下細(xì)胞極少分裂或不分裂。 這些細(xì)胞具有分化為表達(dá)造骨細(xì)胞表型的成熟造骨細(xì)胞的能力。 這些細(xì)胞提供了一個同質(zhì)化的快速增殖模型系統(tǒng),用于研究正常人造骨細(xì)胞的分化,造骨細(xì)胞生理和激素,生長因子,和對造骨細(xì)胞的功能及分化有影響的細(xì)胞因子。
DMEM/F12(1:1): Ham's F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 胎牛血清,10%;0.3mg/ml G418
消化3-5分鐘。1:2。3天內(nèi)可長滿。
不詳
*培養(yǎng)基+8%DMSO
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