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細(xì)胞中文名稱(chēng)小鼠黑色素瘤細(xì)胞
細(xì)胞英文名稱(chēng)B16-F1
物種人
細(xì)胞形態(tài)特征上皮細(xì)胞樣
細(xì)胞生長(zhǎng)特性小鼠黑色素瘤細(xì)胞B16-F1細(xì)胞 貼壁生長(zhǎng)
細(xì)胞特種特性該細(xì)胞源自黑色素瘤C57BL/6J荷瘤小鼠的皮膚,可產(chǎn)生黑色素
培養(yǎng)基DMEM-H: Dulbecco's Modified Eagle's Medium (DME H-21 4.5g/Liter Glucose
血清10% FBS
細(xì)胞傳代方法1:10傳代,每周換液2~3次
細(xì)胞傳代情況C2
細(xì)胞凍存條件基礎(chǔ)培養(yǎng)基+8%DMSO+20%FBS
支原體檢測(cè)培養(yǎng)法(-)
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